The cell-free protein synthesis (CFPS) that synthesizes mRNA and protein from a template DNA has been featured as an important tool to emulate living systems in vitro.However, an obstacle to emulate living cells by CFPS is the loss of activity in the case of usage of high concentration cell extracts.In this study, we found that a high concentration
Abstract Background Mycoheterotrophic plants are one of the most difficult plant groups to conserve because they are entirely dependent on symbiotic fungi.Establishment of viable culture systems would greatly aid their conservation.We describe a simple culture system for the mycoheterotrophic orchid, Gastrodia pubilabiata, that does not require lab
BackgroundPractice variation in healthcare is a complex issue.We focused on practice variation in induction of labor between maternity care networks in the Netherlands.These collaborations of hospitals and midwifery practices are jointly responsible for providing high-quality maternity care.We explored the association between induction rates and ma